ANTIBACTERIAL AND WOUND HEALING OF BIOSYNTHESIZED ZNNPS USING  SESAMUM INDICUM SEEDS AGAINST MULTI DRUG RESISTANT PEPTONIPHILUS HAREI IN ERBIL CITY, IRAQ

Mahabad Mohsin Abdullah; Payman Akram Hamasaeed; Khadija Khalil Barzani

doi:10.46903/gjms/23.2.1779

ANTIBACTERIAL AND WOUND HEALING OF BIOSYNTHESIZED ZNNPS USING SESAMUM INDICUM SEEDS AGAINST MULTI DRUG RESISTANT PEPTONIPHILUS HAREI IN ERBIL CITY, IRAQ

Mahabad Mohsin Abdullah, Payman Akram Hamasaeed, Khadija Khalil Barzani

Abstract

Background: Wound infections are a significant cause of death in patients, particularly those caused by Multidrug resistant (MDR) pathogens. To counter such a danger, new antibacterial agents like nanoparticles (NPs) are needed. The goal of this research was to identify a different treatment drug to fight the isolates of MDR Peptoniphilus harei.

Materials & methods: A total of 157 clinical specimens were obtained from various wounds and hospitals located in Erbil, Iraq, between July and October of 2022. 61 (38.21%) Gram positive bacteria (GPB) were found; based on 16S rRNA (PCR), sequencing, and the GenBank database, one (1.6%) of these was newly reported in Iraq. According to the findings of a sensitivity test conducted on 20 antimicrobials, P. harei was resistant to 14 of them. Using an albino wistar rat model, zinc nanoparticles (ZnNPs) derived from Sesamum indicum seeds were biosynthesized and evaluated against isolated In vitro antibacterial and In vivo wound healing.

Results: According to the current data, ZnNPs’ inhibition zone has 16 mm of antibacterial activity against P. harei. With the standard therapies except Mebo, the recovery period with ZnNPs creams was noticeably faster than that of usual therapy. Wounds infected with P. harei that were treated with Zn NP, Vaseline, Fusidin, and Mebo healed in 22, 27, 28, and 16 days, respectively and the non-treated group took 33 days.

Conclusion: The recent work is a significant step towards creating innovative nanoparticles that provide better alternative uses for antibacterial medications and wound treatments.

Keywords

Antimicrobial activity; Peptoniphilus harei; Sesamum indicum seeds; Wound healing; ZnNPs.

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DOI: https://doi.org/10.46903/gjms/23.2.1779