EFFECT OF TIME INTERVALS ON S. MUTANS AND  IL-2 LEVELS FOR PATIENTS WEARING REMOVABLE  ORTHODONTICS

Rasha jasim Alwarid; Kasem Ahmed Abeas

EFFECT OF TIME INTERVALS ON S. MUTANS AND IL-2 LEVELS FOR PATIENTS WEARING REMOVABLE ORTHODONTICS

Rasha jasim Alwarid, Kasem Ahmed Abeas

Abstract

Background: Streptococcus mutans is the chief organisms in dental plaque that predispose to caries initiation especially in orthodontic wearer. The study objectives are to distinguish and quantify S. Mutans by a molecular (qPCR) technique and estimation the level of IL2 in a group of Iraqi sample wearing removable orthodontic appliance.

Methods: Sixty patients wearing removable orthodontic appliance (36 females and 24 male) with age ranged (14-30) are chosen for sampling procedure. Using different follow up protocol, the study sample was further subdivided into three phases ( T0, T1, T2), representing the (immediate, three weeks, six weeks) time elapsed after removable appliance insertion within the patient's mouth, respectively. Accordingly, from each patient a specimen was taken from the gingival crevicular fluid (GCF) of the proximal surfaces of 1^st molars by utilizing sterilized paper point. In addition about 2-3 ml of unstimulated saliva was also taken from each patient which are needed for evaluating IL-2 by ELIZA .

Results: The S. Mutans mean values are increased significantly from initial insertion time to the 1^st and 2^nd appointment following the appliance wearing. Moreover, the levels of IL-2 are significantly elevated at T1 and T2 of orthodontic treatment, (P- value ≤ 0.001).

Conclusion: Due to the inflammatory role of IL-2 and infectious mechanism of S. Mutans; the study result also signify the direct impact of removable appliance on oral and dental health, unless efficient oral hygiene measures are undertaken by orthodontic patients.

Keywords

Real Time PCR; S. Mutans; IL-2; Removable Orthodontic Appliance

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