DOSE-DEPENDENT PROTECTIVE EFFECTS OF SESAMIN AGAINST RENAL INFLAMMATION AND OXIDATIVE STRESS IN CISPLATIN-TREATED RATS: AN EXPERIMENTAL STUDY

Kumayl Abbas Meghji; Samia Siddiqui; Safiya Javed; Ali Abbas Thalho; Raima Kalhoro; Ahsan Aslam Memon

doi:10.46903/gjms/23.1.Special.1844

DOSE-DEPENDENT PROTECTIVE EFFECTS OF SESAMIN AGAINST RENAL INFLAMMATION AND OXIDATIVE STRESS IN CISPLATIN-TREATED RATS: AN EXPERIMENTAL STUDY

Kumayl Abbas Meghji, Samia Siddiqui, Safiya Javed, Ali Abbas Thalho, Raima Kalhoro, Ahsan Aslam Memon

Abstract

Background: Cisplatin, widely used for treating cancers, often induces nephrotoxicity, limiting clinical use. Sesamin, a lignan from sesame seeds, shows antioxidant and anti-inflammatory effects that may protect renal function. The objective of this study was to assess the dose-dependent anti-oxidative and anti-inflammatory effects of sesamin on cisplatin-induced nephrotoxicity.

Materials & Methods: This quasi-experimental study was performed at Isra University Hyderabad between April and October 2024. Forty male albino Wistar rats were grouped into: Group A (control), Group B (cisplatin 20 mg/kg intraperitoneally), Group C (cisplatin 20 mg/kg intraperitoneally + sesamin 10 mg/kg orally for 10 days), and Group D (cisplatin 20 mg/kg intraperitoneally + sesamin 20 mg/kg orally for 10 days). Following treatment, blood and kidney tissue samples were collected for biochemical and histological analysis. Data were analyzed in SPSS with significance at P ≤ 0.05.

Results: Cisplatin treatment caused significant weight loss (p<0.05) and elevated serum urea, creatinine, inflammatory markers (IL-1, IL-6, TNF-α), and oxidative stress markers (MDA) in Group B (p<0.05). Groups C and D exhibited less pronounced elevations in these parameters. Group D showed the most favorable results, with near-normal histological architecture and improved antioxidant enzyme levels (SOD, GPx), compared to group B (p<0.05). Histopathological analysis revealed severe renal damage in Group B, while groups C and D displayed less pronounced renal injury, with Group D showing the best preservation of kidney structure.

Conclusion: Sesamin demonstrated dose-dependent protection against cisplatin-induced nephrotoxicity by reducing oxidative stress, inflammatory markers, and improving renal function.

Keywords

Cisplatin; Inflammation; Nephrotoxicity; Oxidative Stress; Sesamin.

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DOI: https://doi.org/10.46903/gjms/23.1.Special.1844