EFFECT OF PREEMPTIVE REGIMEN ON C-FOS EXPRESSION IN DORSAL ROOT GANGLION OF RAT UTERINE SURGICAL PAIN MODEL

Iqra Khadam; Najma Baseer; Saima Mumtaz; Meh Laq Amjad; Ayesha Karim Karim; Shabnam Wazir

doi:10.46903/gjms/23.2.1903

EFFECT OF PREEMPTIVE REGIMEN ON C-FOS EXPRESSION IN DORSAL ROOT GANGLION OF RAT UTERINE SURGICAL PAIN MODEL

Iqra Khadam, Najma Baseer, Saima Mumtaz, Meh Laq Amjad, Ayesha Karim Karim, Shabnam Wazir

Abstract

Background: In animal surgical models, the preemptive regime has been shown to be an effective part of balanced anaesthesia with regard to pain-free postoperative recovery. The study investigated the impact of pre-emptive analgesic regimens on pain and c-fos expression in the spinal dorsal root ganglion (1st order neuron) following uterine surgery in rats.

Materials & Methods: The Khyber Medical University in Peshawar’s Ethical Committee of gave its approval to the study protocol (ASRB/IBMS/IRBE/4TH meeting/2023/9821-18, dated 03/04/23). The study was conducted in KMU Peshawar (April 2023 to January 2024). Eighteen Sprague-Dawley adult female rats were used in the experiments (calculated by resource equation) and divided into two study groups: Superficial and Deep pain groups, each group further divide into three study groups: Buprenorphine +Lidocaine, Tramadol+ Lidocaine, Saline under general anesthesia. By using ANOVA, post hoc analysis and Bonferroni and Holm correction, the mean and standard deviation for numerical variables were calculated. The 95% confidence interval corresponded to a standard deviation of 1.96. SPPS version 22 and MS Excel were used to conduct the statistical analysis.

Results: The results showed that the groups treated with buprenorphine or tramadol had significantly reduced c-fos expression, indicating lower pain levels compared to the saline group p>0.05 in both study groups.

Conclusion: Preemptive regime appeared to suppress c-fos expression in DRG 1st order neurons of Spinothalamic tract in uterine pain surgical model. Among the two analgesics compared, buprenorphine appeared to be more potent in suppressing c-fos than tramadol.

Keywords

Buprenorphine; c-fos expression; dorsal root ganglion; image J; immunohistochemistry; Preemptive regimen; postoperative pain; tramadol.

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DOI: https://doi.org/10.46903/gjms/23.2.1903