DIAGNOSTIC IMPORTANCE OF LIVER-TYPE FATTY ACID-BINDING PROTEIN AND DICKKOPF-RELATED PROTEIN 3 IN THE PROGRESSION OF CHRONIC KIDNEY DISEASE

Bakhtawar Farooq; Khawar Khawar Younas; Zahid Habib Qureshi; Mir Mehrab Jan Khetran; Khujasta Gul; Madeeha Shahzad Lodhi

doi:10.46903/gjms/23.4.2102

DIAGNOSTIC IMPORTANCE OF LIVER-TYPE FATTY ACID-BINDING PROTEIN AND DICKKOPF-RELATED PROTEIN 3 IN THE PROGRESSION OF CHRONIC KIDNEY DISEASE

Bakhtawar Farooq, Khawar Khawar Younas, Zahid Habib Qureshi, Mir Mehrab Jan Khetran, Khujasta Gul, Madeeha Shahzad Lodhi

Abstract

Background: Chronic kidney disease (CKD) is a growing global health issue throughout the world. Serum biomarkers can improve CKD diagnostics by revealing early kidney damage. Therefore, study aimed to evaluates the potential of L-FABP and DKK3 biomarkers for early detection of CKD.

Materials & Methods: A case-control study was organized among 200 participants in the Nishtar and Ibn-e-Siena Hospitals Multan, Pakistan from January to December 2022. L-FABP and DKK3 biomarkers were assessed via sandwich enzyme-linked immunosorbent assay (ELISA). Their diagnostic performance was evaluated by receiver–operator curve (ROC) and correlations were analyzed using Pearson coefficient (r). Data were statistically analyzed by one-way analysis of variance (ANOVA).

Results: The present study encompassed a total of 200 participants, comprising 130 individuals diagnosed with kidney disease and 70 healthy control subjects without any prevalent medical conditions. Serum L-FABP was significantly (p<0.0001) higher among AKI (299 ng/L±103), as that of CKD patients (152 ng/L±40), while DKK3 was higher (p<0.0001) in AKI (21 ng/ml±9.8), and CKD (6.6 ng/ml±2.3), compared to controls. ROC shows excellent CKD detection in both biomarkers (AUC > 0.99). Among CKD stages highest level of serum L-FABP and DKK3 was seen in stage-4 and 5 compared to stage-2 and 3. Correlation results found L-FABP shows a strong positive correlation with DKK3 among cases.

Conclusion: Serum L-FABP and DKK3 are effective biomarkers for early CKD detection, which may help in slowing CKD progression.

Keywords

Biomarkers; Chronic Kidney Disease; Diseases; Enzyme-Linked Immunosorbent Assay; Kidney.

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DOI: https://doi.org/10.46903/gjms/23.4.2102