Subhanullah Jan, Shakirullah Jan, Muhammad Jan


Background: Peptic ulcer is mostly produced due to over production of gastric acid. This study was undertaken to compare the effects of extract from fruits of medicinal plant Ammi visnaga which contains natural calcium channel blocker and verapamil on volume and acidity of carbachol induced gastric secretion.
Material & Methods: Thirty rabbits of local breed, weighing 1-1.5 kg were used. The animals were kept fasting for 48 hours, after which the pylorus of each animal was ligated. verapamil 10 mg/kg body weight, Ammi visnaga 500 mg/kg body weight and carbachol 600 mg/kg body weight were administered intraperitoneally.
Results: It was found that verapamil reduced the volume, free and total acidity of gastric secretion, which were statistically highly significant when compared with carbachol (p<0.001). The extract also had the same effects. When the difference of mean for verapamil was compared with that of extract, it was found statistically non-significant indicating that the extract has similar effect as that of verapamil on all parameters included in study i.e. free, combined and total acidity.
Conclusion: The extract of Ammi visnaga can be used effectively and safely in the treatment of hyperacidity conditions and peptic ulcer after evaluation in humans.


Ammi visnaga; Verapamil; Gastric secretion.

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Edward CRW, Bouchier IAD, Haslett C. Diseases of the stomach. In: Davidson’s Principles and Practice of Medicine, Churchill Livingstone, London, 1995. p. 425-34.

Azhar I, Aftab K, Usmanghani K. Naturally occurring calcium channel blockers Hamdard Medicus, 1995; 38:5-16.

Rauwald HW, Brehm O, Odenthal KP. The involvement of a calcium channel blocking mode of action in the pharmacology of Ammi visnaga fruits. Planta Med 1994; 60:101-5.

Rauwald HW, Brehm O. Screening of some medicinal plants for their possible calcium antagonistic activity. Planta Med 1991; 57:A59-A60.

Domiaty-el MM. Improved high-performance liquid chromatographic determination of khellin and visnagin in Ammi visnaga fruits and pharmaceutical formulations. J Pharm Sci 1992;81:475.

Fleckenstein A. History of calcium antagonists. Circ Res 1983; 52:3-16.

Barreras RF. Calcium and gastric secretion. Gastroenterology 1973; 64:1168-84.

Anderson JR. Diseases of the stomach. In: Muir’s Textbook of Pathology, English Language Book Society /Edwardarnold, London, 1985, p.19.18- 19.26.

Basso N, Materia A, Folini A, Jafe BM. Prostaglandin generation in the gastric mucosa of rats with stress ulcer. Surgery 1983; 94:104-8.

Passaro E Jr, Basso N, Walsh, JH. Calcium chal¬lenge in Zollinger Ellision syndrome. Surgery 1972; 72:60-7.

Nandi J, King RL, Kaplan DS, Levine RA. Mechanism of gastric proton pump inhibition by calcium channel antagonists. J Pharamacol Exp Ther 1990; 252:1102-7.

Vischer FE, Seay PH, Tazelaar AP, Veldkamp Jr W, Brook MJ. Pharmacology of famine bromide. J.Pharmacol. Expt Ther 1954; 110:188-204.

Varley, H. Test of gastric function, occult blood. In: Practical Clinical Biochemistry, London, Willium Meinmann, 1962. p 249-77.

Shambuerk RD, Schubert ML. Control of gastric acid secretion. Gastroenterol Clin North Am 1992; 21:527-50.

Negulescu PA, Matchen TE. Intracellular calcium regulation during secretogogues stimulation of the parietal cells. Am J Physiol 1988; 254:130-40.

Brogden RN, Carmine AA, Heel RC, Speight TM, Avery GS. Ranitidine: a review of its pharmacology and therapeutic use in peptic ulcer disease and other allied diseases. Drug 1982; 24;267-303.

Brage R, Cortijo J, Esplugues JK, Esplugues J, Bomnati EM, Rondriguez, Effects of calcium channel blockers on gastric emptying and acid secretion of the rat in vivo. Br J Pharmacol 1986; 89:627-33.

Kirkegaard P, Charistianson J, Peterson B, Olsen PS. Calcium and stimulus-secretion coupling in gastric fundic mucosa: effect of inhibition of calcium transport by Verapamil on gastric acid secretion in the isolated guinea pig fundic mucosa and in healthy subjects. Scand J Gastroenterol 1982; 17:533-8.

Rogers C, Pihan G, Szabo S. Role of leukotrienes in the pathogeneis of haemorrhagic gastric mucosal lesions induced by ethanol or HCL in the rat. Gastroenterol 1986; 90:1797.

Main IHM, Pearce JB. Effects of calcium on acid secretion from the rat isolated gastric mucosa during stimulation with histamine, pentagastrin, methacholine, and dibutyrl cyclic adenosine-3,5-monophosphate. Br J Pharmacol 1978; 64:359-68.

Flekanstein A. Specific pharmacology of calcium in pericardium, cardiac pacemaker and vascular smooth muscles. Ann Rev Pharmacol Toxicol 1977; 17:149-66.

Latif A, Aamir K, Ali M, Siddiqi N. Deactivation of ripened uterine tissue by calcium channel blocker Verapamil. Pak J Pharmacol 2002; 19:13-7.


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