ASSESSMENT OF RELATIONSHIP OF CIRCULATING NITRATE-NITRITE RATIO WITH FEMALE SEX STEROIDS IN DIFFERENT PHASES OF MENSTRUAL CYCLE

Sharmistha Chatterjee, Mousumi Mukhopadhyay

Abstract


Background: Nitric oxide is involved in diverse physiological and pathological conditions, including those of the female reproductive tract. As it has a very short half-life, it is measured as nitrate-nitrite ratio. This study was aimed to assess the interrelationship between the fluctuating levels of estrogen, progesterone and serum nitrate-nitrite ratio in different phases of menstrual cycle measured in healthy females of reproductive age group.
Material & Methods: In this cross-sectional study, blood samples were collected from 71 healthy female volunteers on 3rd, 14th and 28th day of menstruation. The serum estradiol and progesterone were measured by ELISA and nitrate-nitrite ratio by the cadmium reduction method.
Results: Statistics revealed a significant rise (p<0.05) of the nitrate-nitrite ratio along with estrogen. There was also a considerable rise in progesterone in the follicular phase which fell off abruptly with ovulation. But no such rise in the nitrate-nitrite ratio was observed in the luteal phase.
Conclusion: The increase in ratio was analogous to the fluctuations in estrogen throughout the cycle, but no such significant relation with progesterone was observed.

Keywords


Estradiol; Menstrual cycle, Follicular phase; Menstrual cycle, Luteal phase; Nitric oxide; Progesterone.

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References


Rosselli M, Keller PJ, Dubey RK. Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum Reprod Update 1998; 4:3-24.

Yallampalli C, Izumi H, Byam-Smith M, Garfield E. An L-arginine nitric oxide cyclic guanosine monophosphate system exists in the uterus and inhibits contractility during pregnancy. Am J Obstet Gynecol 1994; 170:175-85.

Wieser F, Gruber DM, Tschuggel W, Huber JC. Progesterone and nitric oxide systems. Zentralblatt Fur Gynakologie 1997; 119 Suppl 2:12-6.

Moncada S, Higgs A. The L-arginine-nitric oxide pathway. N Engl J Med 1993; 329:2002-12.

Tsikas D..Methods of quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological fluids. Free Radic Res 2005; 39:797-815.

Moncada S, Palmer RM, Higgs EA. The discovery of nitric oxide as the endogenous nitrovasodilator. Hypertension 1998; 12:365-72.

Jungersten L, Edlund A, Petterson AS, Wennmalm A. Plasma nitrate as an index of nitric oxide formation in man: analyses of kinetics and confounding factors. Clin Physiol 1996; 16:369-79.

Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem 1990; 36:1440-3.

Coughlan T, Gibson C, Murphy S. Modulatory effects of progesterone on inducible nitric oxide synthase expression in vivo and in vitro. J Neurochem 2005; 93:932-42.

Rosselli M, Imthurm B, Macas E, Keller PJ, Dubey RK. Circulating nitrite/nitrate levels increase with follicular development: indirect evidence for es¬tradiol mediated NO release. Biochem Biophys Res Commun 1994; 202:1543-52.

Buhimschi I, Yallampalli C, Dong YL, Garfield RE. Involvement of a nitric oxide cyclic guano¬sine pathway in control of human contractility during pregnancy. Am J Obstet Gynecol 1995; 172:1577-84.

Adkisson EJ, Cassey DP, Beck DT, Gurovich AN, et al. Central, peripheral and resistance arterial reactivity fluctuates during the phases of the menstrual cycle. Exp Biol Med 2010; 235:111-8.

Neudling S, Kahlert S, Loebbert K, Doevendans PA, et al. 17 β-Estradiol stimulates expression of endothelial and inducible NO synthase in rat myocardium in vivo and in vitro. Cardiovasc Res 1999; 43:666-74.

Duncan AC, Petrie JR, Brosnan MJ, Devlin AM, Bass RA, et al. Is cardioprotection a nitric oxide-mediated effect? Human Reprod 2002l; 17:1918-24.

Kubes P, Suzuki M, Granger DN. Nitric oxide: an endogenous modulator of leucocyte adhesion. Proc Natl Acad Sci USA 1991; 88:4651-5.

Stabile LP, Davis ALG, Gubish TC, Hopkins TM, et al. Human non-small cell lung tumors and cells derived from normal lung tissue express both estrogen receptors α and β and show biological response to estrogen. Cancer Res 2002; 62:2141-50.

Reveneau S, Arnould L, Jolimoy G, Hilpert S. Nitric Oxide Synthase in human breast cancer is associated with tumor grade, proliferation rate and expression of progesterone receptors. Lab Invest 1999; 79:1215-25.

Cicinelli E, Ignarro LJ, Matteo MG, Galantino P, et al. Effects of estrogen replacement therapy on plasma levels of nitric oxide in post-menopausal women. Am J Obstet Gynecol 1999; 180:334-9.

Cevik D, Unay O, Durmusoglu F, Yurdun T, et al. Plasma markers of Nitric Oxide synthase activity after ovarian hyperstimulation: influence of estradiol on ADMA. Vasc Med 2006; 11:7-12.


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