Niaz Hussain Soomro, Saifullah Baig, Nisar Rao, Aneeqa Ahsan Zafar, Farwa Hashmat, Sattar Bakhsh Awan, Hashmat Ullah


Background: Tuberculosis is rapidly becoming a global disease. The objective of the study was to determine the yield of the GeneXpert in the detection of DNA of mycobacterium tuberculosis and rifampicin resistance in cervical lymph node tissue specimens.
Material & Methods: This cross-sectional study was conducted at the departments of Chest Medicine and Thoracic Surgery at OJHA Institute of Chest Diseases, Dow University of Health Sciences Karachi, Pakistan, from April 2014 to June 2015. The sample size was 54, selected through non-probibilty consecutive sampling technique. All patients who presented with cervical lymph adenopathy were included in the study. Demographic variables were; gender and age in years. Gender was nominal whereas age was numeric variable. Research variables were; DNA of mycobacterium TB, Rifampicin resistance. Both were nominal having attributes of positive and negative each. Data was collected and analyzed descriptively as percentages and mean along with range depending upon the data type being categorical or numeric. Descriptive statistic were calculated using SPSS Version 15.
Results: Out of 54 patients, 23(42.50%) were males and 29(53.70%) females. The mean age of the patients was 32.3 years with range of 75 years (8- 83 years). Among the 54 samples tested, the GeneXpert detected the DNA of mycobacterium tuberculosis in 40 samples (74%), whereas 14(26%) were found negative. Out of 40, GeneXpert positive patients, Rifampicin resistance was detected in 2 patients (5%). And 38(95%) were Rifampicin sensitive. One of those 2 patients did not have any prior history of anti-tuberculosis therapy and was diagnosed with primary multidrug resistant tuberculosis where as the other patient being diagnosed with tuberculosis previously and completing a course of anti-tuberculosis therapy.
Conclusion: GeneExpert MTB/RIF is an efficient method to detect mycobacterium TB along with Rifampicin sensitivity in cervical lymph node tissue specimens.


Tuberculosis; Rifampicin; Mycobacterium; Diagnosed; Tissue.

Full Text:



Ullah S, Shah SH, Rehman AU, Kamal A, Begum N, Khan G. Extrapulmonary tuberculosis in lady reading hospital Peshawar,Pakistan:a survey of biopsy results. J Ayub Med Coll Abbottabad 2008;20:43-46.

Zafar H. Diagnosis of mycobacterium TB Infection with GeneXpert test along with rifampicin resistance. J Pak Med Assoc 2015;1:22-6.

Dye C, Scheele S, Dolin P, Pathania V, Raviglione MC. Global Burden of tuberculosis-estimated incidence, prevalence, and mortality by country. JAMA 1999; 282:677-86.

Helb D, Jones M, Story E. Rapid detection of mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J Clin Microbiol 2010;48 :1229-37.

Raj A, Singh N, Mehta P. GeneXpert MTB/RIF assay: a new hope for extrapulmonary tuberculosis. IOSR Journal of Pharmacy 2012;2:83-9.

Raja SJ, Ching L, Xi SJ, Hughes R, Chang W, Wong W, et al. Technology for automated, rapid, and quantitative PCR or reverse transcription-PCR clinical testing. Clin Chem 2005 ;518:82-90.

Handa U, Mundi I, Mohan S. Nodal tuberculosis revisited: a review. J Infect Dev Ctries 2012;6:12-5.

Van Kampen SC, Anthony RM, Klatser PR. The realistic performance achievable with mycobacterial automated culture systems in high and low prevalence settings.BMC Infect Dis 2010;10: 93-6.

Siddiqi K, Lambert ML, WalleyJ. Clinical diagnosis of smear-negative pulmonary tuberculosis in low-income countries: the current evidence. Lancet Infect Dis 2003;3:288-96.

Vadwai V, Boehme C, Nabeta P, Shetty A, Alland D, Rodrigues C. Xpert MTB/RIF: a new pillar in diagnosis of extrapulmonary tuberculosis?. J Clin Microbiol 2011;49:2540-5.

World Health Organization (2010) WHO endorses new rapid tuberculosis test. available:

Boehme C, Nabeta P, Hillemann D, Nicol MP, Shenai S. Rapid molecular detection of tuberculosis and rifampin resistance. New Engl J Med 2010; 363: 1005-15.

Arzu N Z, Sezai T, Cengiz C. Evaluation of the GeneXpert MTB/RIF, assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens. J Clin Microbiol 2011;12:4138-41.

Dorris H, Sabine RG, Catherina B. Rapid molecular detection of extrapulmonary tuberculosis by the automated geneXpert MTB/RIF system. J Clin Microbiol 2011;4:1202-5.

Karen RS, Hojoon S, Ian S. Xpert® Mtb/Rif assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane Database Syst Rev 2013;31:1-131.


  • There are currently no refbacks.

© 2011 Gomal Journal of Medical Sciences