Fatima Daud, Mohibullah Khan, Farah Deeba, Muhammad Tariq Khan, Muhammad Daud Khan


Background: Glaucoma is an irreversible optic neuropathy that results in a distinctive pattern of vision loss. The objective of this study was to determine the number of trabecular meshwork cells (TMCs) in adult POAG patients from Khyber Pakhtunkhwa, Pakistan.

Materials & Methods: This cross-sectional study was conducted in Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan from 01/07/2016 to 31/12/2016. Trabecular meshwork samples from 75 POAG patients who underwent trabeculectomy were included. After staining with haematoxylin-eosin, nuclei in TMCs were counted under 400X objective. Number of TMCs, sex, age groups and age in years were variables. Sex and age groups were analyzed as count and percentage. Two ratio variables were skewed, hence analyzed by median/quartile (Q2), Q1, Q3 and IQR with 95%CI. Hypothesis was tested with non-parametric one-sample Wilcoxon signed-rank test.

Results: Seventy-five patients included 58 (77.33%) men and 17 (22.67%) women, with 42 (56%) patients in 36-60 years, 25 (33.33%) in 61-70, 8(10.67%) in 71-80 and no (0.0%) patient in 81-90 years. The mean age was 57.53±10.86 (35-78, range 43) years, 95%CI 55.03-60.03. The median (Q2) number of TMCs/mm2 was 260 (214-367, IQR 153), 95%CI 230-291. Null hypothesis was rejected (p<.00001), showing that the number of TMCs in glaucomatous adults of Khyber Pakhtunkhwa, Pakistan was statistically significantly lower than normative sample of non-glaucomatous enucleated eyes.

Conclusion: The number of trabecular meshwork cells in adult POAG patients from Khyber Pakhtunkhwa, Pakistan was significantly lower than normative sample of non-glaucomatous enucleated eyes.


Glaucoma; Primary Open Angle Glaucoma; Optic Neuropathy; Trabeculectomy; Trabecular Meshwork; Adults; Population; Pakistan.

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