Alaq Ali Abdul Hussein, Masar Riyadh Rashid Almousawi, May Mohammed Ali


Background: It has been determined that Escherichia coli is the prevailing uropathogen (50-90%) in both complicated and uncomplicated urinary tract infections. Uropathogenic Escherichia coli (UPEC) possesses an extensive array of virulence factors, which confers the potential to induce urinary tract infections and is correlated with the development of antibiotic resistance. The purpose of this research was to detect the distribution of virulence genes and their association with antibiotic resistance rates at the molecular level.

Materials & Methods: This study was conducted in the microbiology laboratory at Al-Hussein Medical city teaching hospital, Laboratory of microbiology in Karbala province, Iraq. Urine samples were collected b/w October 2023 to January 2024, and transported for further analysis using standard microbiological and biochemical techniques. The sampling technique used in the study can be described as convenience sampling. The identification of E.coli isolates was confirmed using 16rRNA, resistance and sensitivity to various antibiotics were determined with Vitek2 system. Identification of genes (fimH, KpsmII, Pap, ompT) by uniplex PCR.

Results: E.Coli was shown to be the most often isolated bacterium for various urine samples, with a percentage of 16(35.5%).The most virulent gene observed were Fim H, KpsMII, pap and OmpT were16 (100%), 13 (81.3%), 9(56.3%) and 8(50%) respectively. Thirteen type of antibiotics were identified sensitive to E.coli isolate the most common resistant rate was Ticarcillin, Pipraciilin had high resistance rate 14(87.5), Ciprofloxacin 9(56.3%), Minocycline, Aztreonam, Trimethoprim had rate of 8(50) . Sensitivity rate of Meropenem, Imipenem, Gentamicin was 14(87.5), Cefepime 13(81.25),Amikacin 12(75.0).

Conclusion: The study concluded that there is distribution of virulence gene in E.coli isolates , specially fimH,pap correlated with resistance to antibiotics specially with MDR and XDR patterns.


Antibiotic sensitivity; MDR; Uropathogenic E.coli; Virulence gene; XDR.

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